p73 Antibody (5B429) - BSA Free

Novus Biologicals | Catalog # NBP2-24737

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B429

Format

BSA Free
View all p73 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.

Specificity

p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for p73 Antibody (5B429) - BSA Free

Western Blot: p73 Antibody (5B429)BSA Free [NBP2-24737]

Western Blot: p73 Antibody (5B429)BSA Free [NBP2-24737]

p73-Antibody-5B429-Western-Blot-NBP2-24737-img0009.jpg
Immunocytochemistry/ Immunofluorescence: p73 Antibody (5B429) - BSA Free [NBP2-24737]

Immunocytochemistry/ Immunofluorescence: p73 Antibody (5B429) - BSA Free [NBP2-24737]

Immunocytochemistry/Immunofluorescence: p73 Antibody (5B429) [NBP2-24737] - p73 was detected in immersion fixed Hela human cell line using p73 [5B429] Monoclonal Antibody (Catalog # NBP2-24737) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and in the nuclei.
Immunohistochemistry-Paraffin: p73 Antibody (5B429) - BSA Free [NBP2-24737]

Immunohistochemistry-Paraffin: p73 Antibody (5B429) - BSA Free [NBP2-24737]

Immunohistochemistry-Paraffin: p73 Antibody (5B429) [NBP2-24737] - FFPE breast cancer probed with p73 antibody at 5 ug/ml. Human tissue was used for this test. Staining of tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: p73 Antibody (5B429) - BSA Free [NBP2-24737]

Flow Cytometry: p73 Antibody (5B429) - BSA Free [NBP2-24737]

Flow Cytometry: p73 Antibody (5B429) [NBP2-24737] - Detection of p73 in Human HeLa Cell Line. Human HeLa cell line was stained with Mouse Anti- p73 Monoclonal Antibody (Catalog # NBP2-24737, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Western Blot: p73 Antibody (5B429)BSA Free [NBP2-24737]

Western Blot: p73 Antibody (5B429)BSA Free [NBP2-24737]

Western Blot: p73 Antibody (5B429) [NBP2-24737] - Analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate at 2 ug/ml.
Flow (Intracellular): p73 Antibody (5B429) - BSA Free [NBP2-24737]

Flow (Intracellular): p73 Antibody (5B429) - BSA Free [NBP2-24737]

Flow (Intracellular): p73 Antibody (5B429) [NBP2-24737] - An intracellular stain was performed on HeLa cells with pan p73 (5B429) antibody NBP2-24737 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Simple Western: p73 Antibody (5B429)BSA Free [NBP2-24737]

Simple Western: p73 Antibody (5B429)BSA Free [NBP2-24737]

Simple Western: p73 Antibody (5B429) [NBP2-24737] - Lane view shows a specific band for p73 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for p73 Antibody (5B429) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:20-1:1000

Flow Cytometry

1 ug/mL. Use reported in scientific literature (PMID 21152443)

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Frozen

5-15 ug/ml

Immunohistochemistry-Paraffin

5-15 ug/ml

Immunoprecipitation

1:10-1:500

Simple Western

1:25

Western Blot

2 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:25, apparent MW was 49 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p73

p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (alpha, beta, and gamma) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).

Long Name

Tumor Protein p73

Alternate Names

TP73

Gene Symbol

TP73

UniProt

O15350 (Human)

Additional p73 Products

Product Documents for p73 Antibody (5B429) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for p73 Antibody (5B429) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p73 Antibody (5B429) - BSA Free

Customer Reviews for p73 Antibody (5B429) - BSA Free

There are currently no reviews for this product. Be the first to review p73 Antibody (5B429) - BSA Free and earn rewards!

Have you used p73 Antibody (5B429) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for p73 Antibody (5B429) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Also, have you guys mapped the epitope?

    A: While we know the general region where the epitope lies, we have not yet mapped the exact epitope.

  • Q: Do you know if it also detects deltaN isoforms?

    A: For product NPB2-24737, the immunogen was the full length human p73 protein isoform alpha. The epitope for this product has been mapped to the N-terminal region of this isoform. This region shares 100% homology with isoform delta, so it should also recognize this isoform as well.

  • Q: Also, have you guys mapped the epitope?

    A: While we know the general region where the epitope lies, we have not yet mapped the exact epitope.

  • Q: Do you know if it also detects deltaN isoforms?

    A: For product NPB2-24737, the immunogen was the full length human p73 protein isoform alpha. The epitope for this product has been mapped to the N-terminal region of this isoform. This region shares 100% homology with isoform delta, so it should also recognize this isoform as well.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies