PA Janelia Fluor® 646, SE

Catalog # Availability Size / Price Qty
6150/300U

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PA Janelia Fluor® 646, SE | CAS No. 2093120-32-0 | Photoactivatable Dyes
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Description: Red photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging

Chemical Name: 2,5-Dioxo-1-pyrrolidinyl 3,7-di-1-azetidinyl-2'-diazo-5,5-dimethyl-2',3'-dihydro-5H-3'-oxospiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxylate

Purity: ≥90%

Product Details
Citations (1)
Reviews

Biological Activity

PA Janelia Fluor® 646, SE is a photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor® 549, SE (Cat. No. 6149) to perform two-colour sptPALM in live cells with activation at 405nm. Cell permeable. Excitation maximum = 649 - 651 nm; emission maximum = 663 - 665 nm. Upon protein conjugation the efficiencies of photoconversion improve substantially. Low background staining.

Please see the protocol for further information on protein/antibody labeling and conjugation.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

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Technical Data

M.Wt:
617.74
Formula:
C34H31N5O5Si
Solubility:
Soluble to 20 mM in DMSO
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
2093120-32-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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Citation for PA Janelia Fluor® 646, SE

The citations listed below are publications that use Tocris products. Selected citations for PA Janelia Fluor® 646, SE include:

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