PA28 Activator alpha Subunit/PSME1 Antibody

Novus Biologicals | Catalog # NB100-59751

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PA28 Activator alpha Subunit Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KNFEKLKKPRGETK corresponding to C-Terminus according to NP_006254.1.

Epitope

KNFEKLKKPRGETK

Specificity

This antibody is expected to recognise isoform 1 (NP_006254.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PA28 Activator alpha Subunit/PSME1 Antibody

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NB100-59751]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NB100-59751]

Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody [NB100-59751] - Analysis of PSME1 (isoform 1) using this antibody at 0.3 ug/ml in Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer).

Applications for PA28 Activator alpha Subunit/PSME1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human lymph node, spleen, tonsil and peripheral blood mononuceocyte lysates (calculated MW of 28.7 kDa band according to NP_006254.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PA28 Activator alpha Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 1

Alternate Names

11S Regulator Complex Subunit alpha, IFI5111, PSME1, REG alpha

Entrez Gene IDs

5720 (Human); 19186 (Mouse); 29630 (Rat)

Gene Symbol

PSME1

UniProt

Q06323 (Human)

Additional PA28 Activator alpha Subunit Products

Product Documents for PA28 Activator alpha Subunit/PSME1 Antibody

Certificate of Analysis

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Product Specific Notices for PA28 Activator alpha Subunit/PSME1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PA28 Activator alpha Subunit/PSME1 Antibody

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