PA28 Activator beta Subunit/PSME2 Antibody (06) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-06553

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 06

Format

Azide and BSA Free
View all PA28 Activator beta Subunit Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PA28 Activator beta Subunit/PSME2 (Accession#: Met1-Tyr239).

Reactivity Notes

No cross-reactivity in ELISA with:

E.coli cell lysate

Specificity

No cross-reactivity in ELISA with: E.coli cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PA28 Activator beta Subunit/PSME2 Antibody (06) - Azide and BSA Free

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553]

Western Blot: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:30 kDa
Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553]

Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553]

Immunoprecipitation: PA28 Activator beta Subunit/PSME2 Antibody (06) [NBP3-06553] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-PSME2 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PSME2 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa

Applications for PA28 Activator beta Subunit/PSME2 Antibody (06) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PA28 Activator beta Subunit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13.

Long Name

Proteasome (Prosome, Macropain) Activator Subunit 2

Alternate Names

MCP Activator, PSME2

Gene Symbol

PSME2

Additional PA28 Activator beta Subunit Products

Product Documents for PA28 Activator beta Subunit/PSME2 Antibody (06) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PA28 Activator beta Subunit/PSME2 Antibody (06) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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