PAF1 Antibody - BSA Free
Novus Biologicals | Catalog # NB600-273
![Western Blot: PAF1 Antibody [NB600-273]](https://resources.rndsystems.com/images/products/PAF1-Antibody-Western-Blot-NB600-273-img0006.jpg "Western Blot: PAF1 Antibody [NB600-273]")
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Scientific Data Images for PAF1 Antibody - BSA Free
Western Blot: PAF1 Antibody [NB600-273]
Western Blot: PAF1 Antibody [NB600-273] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPaf1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: PAF1 Antibody [NB600-273]](https://resources.rndsystems.com/images/products/PAF1-Antibody-Immunoprecipitation-NB600-273-img0007.jpg "Immunoprecipitation: PAF1 Antibody [NB600-273]")
Immunoprecipitation: PAF1 Antibody [NB600-273]
Immunoprecipitation: PAF1 Antibody [NB600-273] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-273 used for IP at 3 ug per reaction. Paf1 was also immunoprecipitated by rabbit anti-Paf1 antibody NB600-274. For blotting immunoprecipitated Paf1, NB600-273 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for PAF1 Antibody - BSA Free
Immunoprecipitation
Western Blot
Formulation, Preparation, and Storage
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Background: PAF1
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional PAF1 Products
Product Documents for PAF1 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for PAF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PAF1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PAF1 Antibody - BSA Free
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Q: We used a PAF1 Antibody (NB600-273) (lot no. A1) and I'm wondering if you can let me know the molar concentration (eg. 1M) and the mass concentration (eg. 1mg/ml) of this particular antibody?
A: This antibody is an immunogen affinity purified whole IgG and because the molecular weight of IgG is 150kDa, the molar concentration of this antibody would be 1.33uM.