PAGE4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85413

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAGE4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSSSSVHDLLVAAMSARVRSRSRGRGDGQEAPDVVAFVAPGESQQEEPPTDNQDIEPGQEREGTPPIEERKVEGDCQEMDLEKTRSERGDGSDVKEKTPPNPKHAKTKEAGDGQP

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24708576).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAGE4 Antibody - BSA Free

Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413]

Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413]

Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413] - Staining in human placenta and endometrium tissues using anti-PAGE4 antibody. Corresponding PAGE4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413]

Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413]

Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413]

Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413]

Immunohistochemistry-Paraffin: PAGE4 Antibody [NBP1-85413] - Staining of human placenta shows high expression.
PAGE4 Antibody

Immunohistochemistry: PAGE4 Antibody [NBP1-85413] -

Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, & TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PAGE4 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: PAGE4 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: PAGE4 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-PAGE4 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for PAGE4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
IHC reported in scientific literature (PMID: 24708576). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAGE4

PAGE4 is a member of the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in prostate and prostate cancer, but is also expressed in other male and female reproductive tissues including testis, fallopian tube, uterus, and placenta, as well as in testicular cancer and uterine cancer. The protein encoded by this gene shares sequence similarity with other GAGE/PAGE proteins, and also belongs to a family of CT (cancer-testis) antigens. [provided by RefSeq]

Alternate Names

CT16.7, G antigen family C member 1, G antigen, family C, 1, GAGEC1GAGE-9, JM-27, P antigen family, member 4 (prostate associated), PAGE-1, PAGE-4FLJ35184, Prostate-associated gene 4 protein, prostate-associated gene protein 4

Entrez Gene IDs

9506 (Human)

Gene Symbol

PAGE4

UniProt

O60829 (Human)

Additional PAGE4 Products

Product Documents for PAGE4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PAGE4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PAGE4 Antibody - BSA Free

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Protocols

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