PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)

Novus Biologicals | Catalog # NBP3-16930

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1T7A5 expressed in HEK293
View all PAK1/2/3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S144 of human PAK1/2/3 (Q13153). YMSFT

Modification

p Ser 144, p Ser 141, p Ser 139

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)

Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Application Notes
Modifications: PAK1 - p Ser 144, PAK2 - p Ser 141, PAK3 - p Ser 139

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK1/2/3

In mammals, there are several identified isoforms of p21-activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously. Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death. Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK. (1-4)

Alternate Names

Alpha-PAK, EC 2.7.11, EC 2.7.11.1, MGC130000, MGC130001, p21 protein (Cdc42/Rac)-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, p65-PAK, PAK-1, PAKalpha, serine/threonine-protein kinase PAK 1, STE20 homolog, yeast

Gene Symbol

PAK1

Additional PAK1/2/3 Products

Product Documents for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)

Certificate of Analysis

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Product Specific Notices for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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