Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PAK1 Primary Antibodies »

Product Specifications

Immunogen

Amino Acids encompassing and including phosphorylated Ser199 and Ser204 of human PAK1. Sequence is highly homologous (greater than 90%) to PAK2 and PAK3.

Reactivity Notes

Human

Modification

p Ser204

Specificity

Recognizes PAK1 phosphorylated on Ser199 and Ser204 (human)/Ser198 and Ser203 (mouse). Immunogen sequence is higly homolgous (greater than 90%) with PAK2 and PAK3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NP_002567

Scientific Data Images for PAK1 [p Ser204] Antibody - BSA Free

Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665]

Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665]

Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.

Applications for PAK1 [p Ser204] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 50% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAK1

p21-activated kinases (PAK) are Ser/Thr kinases, which are activated by, and are effectors of Rho family of GTPases. As such, they are implicated in Actin polymerization and activation of the stress-activated kinase cascades. PAK1 is phosphorylated by cdk5, resulting in its inhibition. PAK2 can be activated by Caspase-cleavage in addition to p21-binding, implicating it in cytoskeletal changes during apoptosis. PAK3 is expressed at high levels in post-mitotic neurons of the developing post-natal cerebral cortex and hippocampus, and is the mutant gene thought responsible for non-syndromic X-linked mental retardation.

Long Name

p21/Cdc42/Rac1-activated Kinase 1

Alternate Names

alpha-PAK

Gene Symbol

PAK1

UniProt

Q13153

Additional PAK1 Products

Product Documents for PAK1 [p Ser204] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PAK1 [p Ser204] Antibody - BSA Free

Relevant Entrez Gene Numbers: NP_002567

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
PDGF Family

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