PAK2 Antibody (6D12) - BSA Free

Novus Biologicals | Catalog # NBP3-26442

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D12 expressed in HEK293

Format

BSA Free
View all PAK2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human PAK2 [UniProt Q13177]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK2 Antibody (6D12) - BSA Free

PAK2 Antibody (6D12)

Immunoprecipitation: PAK2 Antibody (6D12) [NBP3-26442] -

Immunoprecipitation: PAK2 Antibody (6D12) [NBP3-26442] - Immunoprecipitating PAK2 in Raji whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26442 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26442 (2ug) + Raji whole cell lysate (500ug).
Lane 3: Raji whole cell lysate (10ug).
PAK2 Antibody (6D12)

Western Blot: PAK2 Antibody (6D12) [NBP3-26442] -

Western Blot: PAK2 Antibody (6D12) [NBP3-26442] - Positive Western Blot detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue.
All lanes: PAK2 Antibody at 1:2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 59 kDa
Observed band size: 59 kDa
PAK2 Antibody (6D12)

Flow Cytometry: PAK2 Antibody (6D12) [NBP3-26442] -

Flow Cytometry: PAK2 Antibody (6D12) [NBP3-26442] - Overlay histogram showing HepG2 cells stained with PAK2 Antibody (6D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for PAK2 Antibody (6D12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: PAK2

p21-activated kinase 2 (PAK2) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). The ubiquitous PAK-2 is unique among the PAK isoforms because it is also activated through proteolytic cleavage by caspases or caspase-like proteases (5). Full-length PAK-2 is localized in the cytoplasm, whereas the proteolytic PAK-2p34 fragment translocates to the nucleus. Activation of full-length PAK-2 stimulates cell survival, whereas proteolytic activation of PAK-2p34 is involved in programmed cell death (6).

Long Name

p21-activated Kinase 2

Alternate Names

gamma-PAK, PAK65, S6/H4 Kinase

Gene Symbol

PAK2

Additional PAK2 Products

Product Documents for PAK2 Antibody (6D12) - BSA Free

Certificate of Analysis

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Product Specific Notices for PAK2 Antibody (6D12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Intracellular Kinases

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Protocols

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