PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)

Novus Biologicals | Catalog # NBP3-32784

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE57-42 expressed in HEK293
View all PAK4+5+6 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser474 of human PAK4. (Uniprot: O96013)

Modification

p Ser 474, p Ser 560, p Ser 602

Localization

Cytoplasm; Mitochondrion, Cytoplasm, Nucleus; Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)

PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)

Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) [NBP3-32784]

Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) [NBP3-32784] - Western blot analysis of PAK4+5+6 on different lysates with Rabbit anti-PAK4+5+6 antibody (NBP3-32784) at 1/1,000 dilution.

Lane 1: A549 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: MCF7 cell lysate (20 ug/Lane)
Lane 4: HUVEC cell lysate (10 ug/Lane)
Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 ug/Lane)

Predicted band size: 64/81/75 kDa
Observed band size: 64 kDa

Exposure time: 30 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32784) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAK4+5+6

Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.

Alternate Names

EC 2.7.11, EC 2.7.11.1, KIAA1142, p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4

Gene Symbol

PAK4

Additional PAK4+5+6 Products

Product Documents for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)

Certificate of Analysis

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Product Specific Notices for PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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