Pappalysin-1/PAPP-A Antibody (PAPPA/8804R)

Novus Biologicals | Catalog # NBP3-20558

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PAPPA/8804R
View all Pappalysin-1/PAPP-A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (within aa 351-523) of human Pappalysin-1/PAPP-A protein (exact sequence is proprietary)

Localization

Secreted

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human placenta tissue.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Pappalysin-1/PAPP-A Antibody (PAPPA/8804R)

Pappalysin-1/PAPP-A Antibody (PAPPA/8804R) Pappalysin-1/PAPP-A Antibody (PAPPA/8804R) - Azide and BSA Free

Pappalysin-1/PAPP-A Antibody (PAPPA/8804R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human placenta stained with PAPP-A Recombinant Rabbit Monoclonal Antibody (PAPPA/8804R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications for Pappalysin-1/PAPP-A Antibody (PAPPA/8804R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pappalysin-1/PAPP-A

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Long Name

Pregnancy-associated Plasma Protein 1

Alternate Names

ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA, Pappalysin1

Gene Symbol

PAPPA

Additional Pappalysin-1/PAPP-A Products

Product Documents for Pappalysin-1/PAPP-A Antibody (PAPPA/8804R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Pappalysin-1/PAPP-A Antibody (PAPPA/8804R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Pappalysin-1/PAPP-A Antibody (PAPPA/8804R)

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  • Q: I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).

    A: Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

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