PARG Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94199

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all PARG Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 777-976 of human PARG (NP_003622.2). HNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEKMRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRDIYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARG Antibody - Azide and BSA Free

Western Blot: PARG AntibodyAzide and BSA Free [NBP2-94199]

Western Blot: PARG AntibodyAzide and BSA Free [NBP2-94199]

Western Blot: PARG Antibody [NBP2-94199] - HCC1937 human breast cancer cells untreated (1) and treated with PARP inhibitor (2) for 96 h. 3 PARG bands 95 kDa can be seen in both cases. Primary antibody dilution 1:1000 (overnight, 4C). Image from verified customer review.
PARG Antibody - Azide and BSA Free

Western Blot: PARG Antibody - Azide and BSA Free [PARG] -

Western Blot: PARG Antibody - Azide and BSA Free [PARG] - Western blot analysis of lysates from PC-3 cells, using PARG Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for PARG Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:100 - 1:500

Reviewed Applications

Read 1 review rated 5 using NBP2-94199 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PARG

Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq]

Long Name

Poly [ADP-ribose] Glycohydrolase

Alternate Names

PARG99

Gene Symbol

PARG

Additional PARG Products

Product Documents for PARG Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PARG Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PARG Antibody - Azide and BSA Free (1)

5 out of 5
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Customer Images

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  • PARG Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells HCC1937
    Species: Human
    Verified Customer | Posted 05/30/2022
    HCC1937 human breast cancer cells untreated (1) and treated with PARP inhibitor (2) for 96 h. 3 PARG bands >95 kDa can be seen in both cases.
    Primary antibody dilution 1:1000 (overnight, 4ºC)
    PARG Antibody - Azide and BSA Free NBP2-94199

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