Human PARP ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-18217

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell lysates, tissue homogenates and other biological fluids (100 ul)

Sensitivity

0.33 ng/ml

Assay Range

0.78-50 ng/ml
View all PARP ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human PARP in the sample, this Human PARP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human PARP concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Precision

Intra-Assay Precision (Precision within an assay) %CV 10

Inter-Assay Precision (Precision between assays) %CV 15

Scientific Data Images for Human PARP ELISA Kit (Colorimetric)

ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217]

ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217]

ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217] - Standard Curve Reference

Kit Contents for Human PARP ELISA Kit (Colorimetric)

  • Balance Solution
  • Enzyme Conjugate
  • Microtiter Plate
  • Standards
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PARP

Poly (ADP-ribose) polymerase (PARP) is protein family primarily invloved in DNA repair and programmed cell death. PARP proteins are activated in response to DNA single strand breaks (SSBs). Upon SSB detection, PARP binds the DNA and synthesizes a PAR chain at the damaged site, which then signals the initiation of DNA repair by other enzymes such as XRCC1, DNA ligase III and DNA polymerase beta. After repair, the PAR chains are degraded via PAR glycohydrolase.

PARP is inactivated by caspase cleavage, leading to a programmed cell death pathway.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Gene Symbol

PARP1

Additional PARP Products

Product Documents for Human PARP ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human PARP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human PARP ELISA Kit (Colorimetric)

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  • Q: Do you have any 100 coda or bigger controls?

    A: I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

  • Q: I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).

    A: For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.

  • Q: Do you have any 100 coda or bigger controls?

    A: I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

  • Q: I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).

    A: For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.

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