PAX8 Antibody (MSVA-708R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57324

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-708R

Format

Azide and BSA Free
View all PAX8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human PAX8 protein Swissprot Q06710

Localization

Nuclear

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Kidney: At least weak to moderate, distinct nuclear staining should be seen in the majority of epithelial cells of the proximal and distal renal tubules, collection ducts, and the parietal epithelial cells of the Bowman's capsule.

Negative Control
Negitve control:Kidney: At least weak to moderate, distinct nuclear staining should be seen in the majority of epithelial cells of the proximal and distal renal tubules, collection ducts, and the parietal epithelial cells of the Bowman's capsule.

Scientific Data Images for PAX8 Antibody (MSVA-708R) - Azide and BSA Free

PAX8 Antibody (MSVA-708R) - Azide and BSA Free

Immunohistochemistry-Paraffin:PAX8 Antibody (MSVA-708R) - Azide and BSA FreeNBP3-57324

Strong nuclear PAX8 staining of a subset of epithelial cells in the fallopian tube
PAX8 Antibody (MSVA-708R) - Azide and BSA Free

Immunohistochemistry-Paraffin:PAX8 Antibody (MSVA-708R) - Azide and BSA FreeNBP3-57324

PAX8 staining is completely absent in lymphocytes lymph node
PAX8 Antibody (MSVA-708R) - Azide and BSA Free

Immunohistochemistry-Paraffin:PAX8 Antibody (MSVA-708R) - Azide and BSA FreeNBP3-57324

Serous high grade ovarian carcinoma with strong PAX8 staining of tumor cells

Applications for PAX8 Antibody (MSVA-708R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAX8

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described.

Alternate Names

paired box 8, paired box gene 8, paired box protein Pax-8, paired domain gene 8

Gene Symbol

PAX8

Additional PAX8 Products

Product Documents for PAX8 Antibody (MSVA-708R) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PAX8 Antibody (MSVA-708R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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