Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-11683

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell Lysate, Cell-based assays (100 ul)

Sensitivity

30 pg/ml

Assay Range

0.25-16 ng/ml
View all PBEF/Visfatin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA (quantitative)

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

Detects human Nampt. Weakly cross-reacts (15%) with mouse Nampt and rat Nampt. Does not cross-react with human adiponectin, human resistin, human RELM-beta or human leptin.

Description

The Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) is to be used for the in vitro quantitative determination of human PBEF/Visfatin/NAMPT in cell lysates or cell-based assays (screening).

Precision

Intra-Assay Precision (Precision within an assay) Sample1: CV = 2.73% (n=10) Sample1: CV = 9.76% (n=10)

Inter-Assay Precision (Precision between assays) Sample1: CV = 4.02% (n=6) Sample1: CV = 7.40% (n=6)

Recovery for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

Recovery

90-105%

Scientific Data Images for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP3-11683]

ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP3-11683]

ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP3-11683] - Sample standard curve

Kit Contents for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

  • 1 bottle Lysis Buffer 10X (12 ml)
  • 1 bottle Stop Solution (12 ml)
  • 1 bottle TMB Substrate Solution (12 ml)
  • 1 plate coated with human PBEF/Visfatin/NAMPT Antibody (6 x 16-well strips)
  • 1 vial Detection Antibody (60 ul)
  • 1 vial HRP 100X (HRP Conjugated anti-rabbit IgG) (150 ul)
  • 1 vial human PBEF/Visfatin/NAMPT Standard (lyophilized) (32 ng)
  • 2 bottles ELISA Buffer 10X (2 x 30 ml)
  • 2 bottles Wash Buffer 10X (2 x 30 ml)
  • 2 plate sealers (plastic film)
  • 2 silica Gel Minibags

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PBEF/Visfatin

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Gene Symbol

NAMPT

Additional PBEF/Visfatin Products

Product Documents for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail