PCBP2 Antibody (5F12) - Azide and BSA Free

Novus Biologicals | Catalog # H00005094-M07

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5F12

Format

Azide and BSA Free
View all PCBP2 Primary Antibodies »

Product Specifications

Immunogen

PCBP2 (AAH01155, 1 a.a. ~ 362 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVKQICVVMLESPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWAGLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMGSS

Reactivity Notes

Rat reactivity reported from customer review.

Specificity

PCBP2 - poly(rC) binding protein 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PCBP2 Antibody (5F12) - Azide and BSA Free

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07]

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07]

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12. Lane 1: PCBP2 transfected lysatE (38.7 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PCBP2 Antibody (5F12) [H00005094-M07]

Immunocytochemistry/ Immunofluorescence: PCBP2 Antibody (5F12) [H00005094-M07]

Immunocytochemistry/Immunofluorescence: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: PCBP2 Antibody (5F12) [H00005094-M07]

Immunohistochemistry-Paraffin: PCBP2 Antibody (5F12) [H00005094-M07]

Immunohistochemistry-Paraffin: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml
Western Blot: PCBP2 Antibody (5F12) [H00005094-M07]

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07]

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi or non-transfected control. Blot probed with H00005094-M07. GAPDH (36.1 kDa) used as loading control.
Western Blot: PCBP2 Antibody (5F12) [H00005094-M07]

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07]

Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in K-562 (Cat # L009V1).
ELISA: PCBP2 Antibody (5F12) [H00005094-M07]

ELISA: PCBP2 Antibody (5F12) [H00005094-M07]

ELISA: PCBP2 Antibody (5F12) [H00005094-M07] - Detection limit for recombinant GST tagged PCBP2 is approximately 0.1ng/ml as a capture antibody.

Applications for PCBP2 Antibody (5F12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB.

Reviewed Applications

Read 1 review rated 3 using H00005094-M07 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCBP2

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of only three have been characterized to date.

Alternate Names

alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, heterogenous nuclear ribonucleoprotein E2, hnRNP E2, hnRNP-E2, HNRPE2, MGC110998, poly(rC) binding protein 2, poly(rC)-binding protein 2

Entrez Gene IDs

5094 (Human)

Gene Symbol

PCBP2

OMIM

601210 (Human)

UniProt

AAH01155 (Human)

Additional PCBP2 Products

Product Documents for PCBP2 Antibody (5F12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PCBP2 Antibody (5F12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PCBP2 Antibody (5F12) - Azide and BSA Free

Customer Reviews for PCBP2 Antibody (5F12) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: human liver cells, Sample Amount: 15 ug
    Species: Human
    Verified Customer | Posted 02/24/2010

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