PCK1 Antibody (JU84-39)

Novus Biologicals | Catalog # NBP2-75609

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU84-39 expressed in HEK293
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Product Specifications

Immunogen

Synthetic peptide within Human PCK1 aa 26-75 / 622. (SwissProt: P35558 Human)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCK1 Antibody (JU84-39)

Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609] - Analysis of paraffin-embedded human kidney tissue using anti-PCK1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609]

Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609] - Analysis of paraffin-embedded human liver tissue using anti-PCK1 antibody. Counter stained with hematoxylin.

Applications for PCK1 Antibody (JU84-39)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCK1

The PCK1 gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expressio

Long Name

Phosphoenolpyruvate Carboxykinase 1

Alternate Names

PEPCK-C, PEPCK1

Gene Symbol

PCK1

Additional PCK1 Products

Product Documents for PCK1 Antibody (JU84-39)

Certificate of Analysis

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Product Specific Notices for PCK1 Antibody (JU84-39)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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