PCK2 Antibody

Novus Biologicals | Catalog # NB100-2422

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PCK2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KPWKPGDKEPCAH corresponding to internal region according to NP_004554.2.

Specificity

This antibody is expected to recognize isoform 1 (NP_004554) only, no cross-reactivity with PCK1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PCK2 Antibody

Western Blot: PCK2 Antibody [NB100-2422]

Western Blot: PCK2 Antibody [NB100-2422]

Western Blot: PCK2 Antibody [NB100-2422] - Staining of NIH3T3 cell lysate with antibody at 2 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PCK2 Antibody [NB100-2422]

Immunocytochemistry/ Immunofluorescence: PCK2 Antibody [NB100-2422]

Immunocytochemistry/Immunofluorescence: PCK2 Antibody [NB100-2422] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: PCK2 Antibody [NB100-2422]

Flow Cytometry: PCK2 Antibody [NB100-2422]

Flow Cytometry: PCK2 Antibody [NB100-2422] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: PCK2 Antibody [NB100-2422]

Western Blot: PCK2 Antibody [NB100-2422]

Western Blot: PCK2 Antibody [NB100-2422] - Staining of A431 (A), HEK293 (B) with antibody at 2 ug/mL and HepG2 (C) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for PCK2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

1 - 3 ug/mL
Application Notes
Approx 70 kDa band observed in lysates of cell lines A431, HEK293 HepG2 and NIH3T3 (calculated MW of 70.7 kDa according to Human NP_004554.3 and 70.5 kDa according to Mouse NP_083270.2).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PCK2

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Alternate Names

EC 4.1.1.32, PE, PEPCK, PEPCK2PEP carboxykinase, PEPCK-M, phosphoenolpyruvate carboxykinase [GTP], mitochondrial, phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase

Entrez Gene IDs

5106 (Human); 74551 (Mouse); 361042 (Rat)

Gene Symbol

PCK2

UniProt

NP_004554.2 (Human)

Additional PCK2 Products

Product Documents for PCK2 Antibody

Certificate of Analysis

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Product Specific Notices for PCK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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