PCM1 Antibody

Novus Biologicals | Catalog # NB100-58828

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PCM1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1925 and 1975 of human pericentriolar material 1 using the numbering given in entry NP_006188.2 (GeneID 5108).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCM1 Antibody

Western Blot: PCM1 Antibody [NB100-58828]

Western Blot: PCM1 Antibody [NB100-58828]

Western Blot: PCM1 Antibody [NB100-58828] - Detection of mouse PCM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: PCM1 Antibody [NB100-58828]

Western Blot: PCM1 Antibody [NB100-58828]

Western Blot: PCM1 Antibody [NB100-58828] - Detection of Human and Mouse PCM1 on HeLa whole cell lysate using NB100-58828. PCM1 was also immunoprecipitated by rabbit anti-PCM1 antibody NB100-58829.
Western Blot: PCM1 Antibody [NB100-58828]

Western Blot: PCM1 Antibody [NB100-58828]

Western Blot: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, A-549, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCM1 antibody NB100-58828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: PCM1 Antibody [NB100-58828]

Immunoprecipitation: PCM1 Antibody [NB100-58828]

Immunoprecipitation: PCM1 Antibody [NB100-58828] - Detection of human PCM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PCM1 antibody NB100-58828 (lot NB100-58828-2) used for IP at 6 ug per reaction. PCM1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-58828-1) and rabbit anti-PCM1 antibody NB100-58829. For blotting immunoprecipitated PCM1, NB100-58828 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for PCM1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCM1

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Alternate Names

hPCM-1, pericentriolar material 1, pericentriolar material 1 protein, PTC4PCM-1

Entrez Gene IDs

5108 (Human); 18536 (Mouse)

Gene Symbol

PCM1

UniProt

Q15154 (Human)

Additional PCM1 Products

Product Documents for PCM1 Antibody

Certificate of Analysis

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Product Specific Notices for PCM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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