Collagen maturation includes the processing of N- and C-propeptides by Procollagen N- and C-proteinases encoded by the ADAMTS2 and BMP-1 genes, respectively. PCPE (Procollagen C-proteinase Enhancer) binds to either side of the C-propeptide cleavage site, thereby facilitating the Procollagen C-proteinase activity of BMP1. PCPE does not enhance the chordinase activity of BMP-1.
PCPE-1/PCOLCE Antibody [Janelia Fluor® 525]
Novus Biologicals | Catalog # AF2627JF525
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Janelia Fluor 525
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PCPE-1
Met1-Asp449
Accession # Q15113
Met1-Asp449
Accession # Q15113
Specificity
Detects human PCPE-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PCPE-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for PCPE-1/PCOLCE Antibody [Janelia Fluor® 525]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: PCPE-1
Long Name
Procollagen C-Proteinase Enhancer-1
Alternate Names
PCOLCE, PCPE1
Gene Symbol
PCOLCE
Additional PCPE-1 Products
Product Documents for PCPE-1/PCOLCE Antibody [Janelia Fluor® 525]
Product Specific Notices for PCPE-1/PCOLCE Antibody [Janelia Fluor® 525]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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