Human PCPE-1 Antibody

R&D Systems | Catalog # AF2627

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PCPE-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PCPE-1
Met1-Asp449
Accession # Q15113

Specificity

Detects human PCPE-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PCPE-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human PCPE-1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture media from NS0 cells transfected with recombinant human PCPE-1, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human PCPE‑1

Reviewed Applications

Read 1 review rated 4 using AF2627 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PCPE-1

PCPE-1 is a 55 kDa extracellular protein that lacks intrinsic enzymatic activity. It functions as a substrate-specific adaptor protein that stimulates C-terminal processing of the fibrillar procollagens I, II and III by PCP (also known as BMP-1/tolloid metalloproteinases). PCPE is naturally processed into N-terminal fragments of 34 and 36 kDas, both of which also enhance PCP. A 16.5 kDa protein corresponding to a C-terminal fragment of PCPE has been described and shown to have metalloproteinase inhibitor activity. Human PCPE-1 shares approximately 81% amino acid sequence homology with mouse and rat PCPE-1.

Long Name

Procollagen C-Proteinase Enhancer-1

Alternate Names

PCOLCE, PCPE1

Entrez Gene IDs

5118 (Human); 18542 (Mouse)

Gene Symbol

PCOLCE

UniProt

Q15113

Additional PCPE-1 Products

Product Documents for Human PCPE-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PCPE-1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human PCPE-1 Antibody

Customer Reviews for Human PCPE-1 Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
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4 Stars
100%
3 Stars
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2 Stars
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1 Stars
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  • Human Persephin Antibody
    Name: Anonymous
    Application: Protein simple using Jess device.
    Sample Tested: Heart lysates
    Species: Human
    Verified Customer | Posted 09/14/2021
    Saturation of AF2627 antibody signal on heart lysates
    Use antibody at 10 fold dilution (saturation of mAb signal). Protein simple using Jess device, validation of antibody.
    Human PCPE-1 Antibody AF2627
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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