PDE5A Antibody

Novus Biologicals | Catalog # NB300-957

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PDE5A Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LINGESGQAKRN corresponding to C-Terminus according to NP_001074.2, NP_246273.2, NP_236914.2, NP_237223.

Specificity

This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE5A Antibody

Western Blot: PDE5A Antibody [NB300-957]

Western Blot: PDE5A Antibody [NB300-957]

Western Blot: PDE5A Antibody [NB300-957] - HEK293 overexpressing PDE5A and probed with (mock transfection in first lane).
Western Blot: PDE5A Antibody [NB300-957]

Western Blot: PDE5A Antibody [NB300-957]

Western Blot: PDE5A Antibody [NB300-957] - (0.3ug/ml) of human lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for PDE5A Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 100 kDa band observed in human lung lysates (calculated MW of 100 kDa band according to isoform 1, NP_001074.2). In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa band according to isoform 2, NP_236914.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE5A

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms.

Long Name

cGMP-specific 3',5'-cyclic phosphodiesterase

Alternate Names

CGB-PDE, PDE5

Entrez Gene IDs

8654 (Human); 171115 (Rat)

Gene Symbol

PDE5A

UniProt

O76074 (Human), NP_001074.2 (Human), NP_246273.2; (Human), NP_236914.2 (Human)

Additional PDE5A Products

Product Documents for PDE5A Antibody

Certificate of Analysis

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Product Specific Notices for PDE5A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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