Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDE8B Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 835 and 885 of human Phosphodiesterase 8B using the numbering given in entry NP_003710.1 (GeneID 8622).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE8B Antibody - BSA Free

Immunoprecipitation: PDE8B Antibody [NBP1-71817]

Immunoprecipitation: PDE8B Antibody [NBP1-71817]

Immunoprecipitation: PDE8B Antibody [NBP1-71817] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE8B antibody used for WB at 1 ug/ml (A and B) and used for IP at 6 ug/mg lysate. PDE8B was also immunoprecipitated by rabbit anti-PDE8B antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Applications for PDE8B Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 5 µg/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDE8B

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 5-prime,3-prime-cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been grouped into several families based on substrate affinities, inhibitor sensitivities, mode of regulation, and amino acid sequence homologies. The PDE8 family contains high-affinity cAMP-specific, IBMX (3-isobutyl-1-methyl-xanthine)-insensitive PDEs. All PDEs share a conserved C-terminal catalytic region and a variable N-terminal domain that presumably accounts for the distinctive regulatory properties unique to the individual families. PDE8B exhibits cAMP PDE activity which was not inhibited by various PDE inhibitors, including IBMX. The cAMP hydrolytic activity was unaffected by cGMP, and no cGMP PDE hydrolysis has been detected. Northern blot analysis indicated that the 4.2-kb PDE8B mRNA was expressed at the highest levels in thyroid, and at lower levels in brain, spinal cord, and placenta.

Long Name

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Alternate Names

HsPDE8B, PIG22

Gene Symbol

PDE8B

UniProt

NP_003710.1 (Human)

Additional PDE8B Products

Product Documents for PDE8B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDE8B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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