Mouse PDGF-AA ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-42754

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (100 uL)

Sensitivity

6.3 pg/mL (example only; lot dependent)

Assay Range

15.6 - 1000 pg/mL (example only; lot dependent)
View all PDGF-AA ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Recovery for Mouse PDGF-AA ELISA Kit (Colorimetric)

Recovery

88 - 99% (example only; lot dependent)

Kit Contents for Mouse PDGF-AA ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PDGF-AA

PDGF-AA (Platelet-Derived Growth Factor AA) is a widely expressed protein that forms disulfide linked homodimers or heterodimers with PDGF-B. PDGF-AA is expressed by monocytes, macrophages, fibroblasts, vascular smooth muscle cells, and vascular endothelial cells. It acts principally as a chemoattractant and inducer of protein synthesis in multiple cell types. PDGF-AA binds and signals through homodimers of the receptor tyrosine kinase PDGF R alpha which itself associates with FGF R1 and Neuropilin-1. PDGF-AA additionally signals through heterodimers of PDGF R alpha and PDGF R beta.

Long Name

Platelet-derived Growth Factor AA

Alternate Names

PDGFAA

Gene Symbol

PDGFA

Additional PDGF-AA Products

Product Documents for Mouse PDGF-AA ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse PDGF-AA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

PDGF Family

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FAQs

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