Human PDGF-BB ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60113

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

7.81 pg/mL

Assay Range

31.25 pg/mL - 2000 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human PDGF-BB. No significant cross-reactivity or interference between human PDGF-BB and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Human PDGF-BB ELISA Kit (Colorimetric)

Human PDGF-BB ELISA Kit (Colorimetric)

Human PDGF-BB ELISA Kit (Colorimetric) [NBP2-60113] -

Human PDGF-BB ELISA Kit (Colorimetric) [NBP2-60113] - Standard Curve Reference

Kit Contents for Human PDGF-BB ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PDGF-BB

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-BB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-BB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Platelet-derived Growth Factor BB

Alternate Names

PDGFBB

Gene Symbol

PDGFB

Additional PDGF-BB Products

Product Documents for Human PDGF-BB ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human PDGF-BB ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

PDGF Family

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