PDK3 Antibody (3A1) - Azide and BSA Free

Novus Biologicals | Catalog # H00005165-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A1

Format

Azide and BSA Free
View all PDK3 Primary Antibodies »

Product Specifications

Immunogen

PDK3 (AAH15948, 174 a.a. ~ 263 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDR

Specificity

PDK3 (3A1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PDK3 Antibody (3A1) - Azide and BSA Free

Western Blot: PDK3 Antibody (3A1) [H00005165-M02]

Western Blot: PDK3 Antibody (3A1) [H00005165-M02]

Western Blot: PDK3 Antibody (3A1) [H00005165-M02] - PDK3 monoclonal antibody (M02), clone 3A1 Analysis of PDK3 expression in A-431.
Sandwich ELISA: PDK3 Antibody (3A1) [H00005165-M02] - Detection limit for recombinant GST tagged PDK3 is approximately 0.03ng/ml as a capture antibody.

Applications for PDK3 Antibody (3A1) - Azide and BSA Free

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reviewed Applications

Read 1 review rated 3 using H00005165-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDK3

PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Long Name

[Pyruvate dehydrogenase

Alternate Names

PDHK3

Entrez Gene IDs

5165 (Human)

Gene Symbol

PDK3

OMIM

602526 (Human)

UniProt

AAH15948 (Human)

Additional PDK3 Products

Product Documents for PDK3 Antibody (3A1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PDK3 Antibody (3A1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PDK3 Antibody (3A1) - Azide and BSA Free (1)

3 out of 5
1 Customer Rating
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  • PDK3 Antibody (3A1)
    Name: Masashi Okada
    Application: Western Blot
    Sample Tested: Human Glioma cells, whole cell lysates
    Species: Human
    Verified Customer | Posted 09/24/2023
    human glioblastoma cell line treated with control or drug and these whole cell lysate (10 ug) were loaded onto SDS-PAGE and subjected to WB. band pointed arrow (46 kDa) is PDK3. primary Ab: 1/1000 dil in 2% BSA TBST for overnight at 4C.
    PDK3 Antibody (3A1) - Azide and BSA Free H00005165-M02

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