PDLIM4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80833

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Mouse (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDLIM4 Antibody - BSA Free

Western Blot: PDLIM4 Antibody [NBP1-80833]

Western Blot: PDLIM4 Antibody [NBP1-80833]

PDLIM4-Antibody-Western-Blot-NBP1-80833-img0015.jpg
Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NBP1-80833]

Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NBP1-80833]

Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP1-80833] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.
Western Blot: PDLIM4 Antibody [NBP1-80833]

Western Blot: PDLIM4 Antibody [NBP1-80833]

Western Blot: PDLIM4 Antibody [NBP1-80833] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Western Blot: PDLIM4 Antibody [NBP1-80833]

Western Blot: PDLIM4 Antibody [NBP1-80833]

Western Blot: PDLIM4 Antibody [NBP1-80833] - Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human Fallopian tube shows weak to moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833]

Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.
PDLIM4 Antibody

Western Blot: PDLIM4 Antibody [NBP1-80833] -

Western Blot: PDLIM4 Antibody [NBP1-80833] - SH-I-14 reduces the in vivo growth of tumors in xenograft mice bearing tumors of MDA-MB-231 cellsA. SH-I-14 (10 mg/kg) or DMSO was injected into peritoneal cavity of mouse with MDA-MB-231 tumors three times per week from 8-days after tumor cell injection. The control mice were euthanized earlier due to large tumor size. Tumor volumes were measured as described in Materials & Methods. Data are shown as mean±SD. **P < 0.01. B. SH-I-14 reduces acetyl-STAT3 & induces the expression of VHL & PDLIM4 protein in vivo. The MDA-MB-231 xenograft mice (3 mice per group) were administered with 10 mg/kg of SH-I-14 daily for 5 days & lysates from each tumor were subjected to western blot analysis as indicated. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29137356), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PDLIM4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 29137356). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDLIM4

PDLIM4, also known as PDZ and LIM domain protein 4, has 2 isoforms, a 330 amino acid long isoform that is 35 kDa and a short 246 amino acid isoform that is 26 kDa and found in brain. May be involved in bone development. Disease research is being performed on this protein involvement in osteoporosis, bronchiolitis, prostate cancer, prostatitis, multiple personality disorder, and asperger syndrome. The protein interacts with RBPMS, PTPN13, TRIP6, MYC, ZNF408, ABCA1, NFE2, SLC2A4, SSBP, TCF3, and ZBTB9 proteins.

Alternate Names

LIM domain protein, LIM protein RIL, PDZ and LIM domain 4, PDZ and LIM domain protein 4, Reversion-induced LIM protein, RILenigma homolog

Entrez Gene IDs

8572 (Human)

Gene Symbol

PDLIM4

UniProt

Additional PDLIM4 Products

Product Documents for PDLIM4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PDLIM4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDLIM4 Antibody - BSA Free

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Protocols

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