Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.9%

Inter-Assay Precision (Precision between assays) CV% 7.1%

Recovery for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

Scientific Data Images for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

Kit Contents for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)

• Biotinylated Human Serum Amyloid P Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against SAP.
• Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine.
• Human Serum Amyloid P Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human SAP.
• Human Serum Amyloid P Standard: Human SAP in a buffered protein base (lyophilized).
• MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
• Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
• Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
• Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
• Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Background: Pentraxin 2/SAP

Serum Amyloid P (SAP) is a non-fibrillar plasma glycoprotein that belongs to the pentraxin family. It is universally found in amyloid deposits and this is probably due to its specific calcium-dependent binding to motifs present on all types of amyloid fibrils. SAP is also found to prevent fibrillar breakdown by enzymes and it is believed that it helps maintains stability of the amyloid deposits (1,2). It has been shown that SAP binds monocytes with high avidity, but does not bind to erythrocytes, NK cells, T lymphocytes or B lymphocytes (3). SAP production can be induced by exposure to IL-1, IL-6 and IFN-beta. The SAP-inducing activity was neutralized by antibodies to each of the recombinant cytokines (4).

Additional Pentraxin 2/SAP Products

• All Products for Pentraxin 2/SAP
• Pentraxin 2/SAP cDNA Clones
• Pentraxin 2/SAP ELISA Kits
• Pentraxin 2/SAP Lysates
• Pentraxin 2/SAP Primary Antibodies
• Pentraxin 2/SAP Proteins and Enzymes

Product Documents for Human Pentraxin 2/SAP ELISA Kit (Colorimetric)