![Western Blot: Pericentrin Antibody [NB100-63208]](https://resources.rndsystems.com/images/products/Pericentrin-Antibody-Western-Blot-NB100-63208-img0001.jpg "Western Blot: Pericentrin Antibody [NB100-63208]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide sequence C-ARAIIREGSLEGS corresponding to the C-terminal of pericentrin 1 (NP_079120)
Reactivity Notes
Predicted cross-reactivities: Mouse, Rat
Localization
Nuclear
Marker
Centrosome Marker
Specificity
NB100-63208 recognizes the C-terminal of human pericentrin 1, a 75 kDa component of the filamentous matrix of the centrosome, expressed in all tissues. When associated with centrosome and Golgi localized protein kinase N-associated protein (CG-NAP), pericentrin 1 anchors the gamma-tubulin ring complex, providing a site for microtubule nucleation and the initial establishment of organized microtubule arrays. It binds calmodulin and is a constituent of nuclear pore proteins (nup) 107-160, a sub-complex of the nuclear pore complex. Pericentrin 1 is thought to play a role in the functioning of the centrosomes, cytoskeleton, and cell-cycle progression.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Pericentrin Antibody - C-terminus
Western Blot: Pericentrin Antibody [NB100-63208]
Western Blot: Pericentrin Antibody [NB100-63208] - Staining of HeLa lysate (35 ug protein in RIPA buffer) using Goat anti Human pericentrin 1 antibody at 1.0 ug/mL. Primary incubation was 1 hour. Signal was detected by chemiluminescence.Applications for Pericentrin Antibody - C-terminus
Application
Recommended Usage
ELISA
>1:32000
Western Blot
1 - 3 ug/mL
Reviewed Applications
Read 1 review rated 1 using NB100-63208 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TRIS buffered saline, 0.5% Bovine Serum Albumin
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pericentrin
Alternate Names
Kendrin, KIAA0402, MOPD2, PCN, PCNT2pericentrin B, PCNTB, pericentrin, pericentrin 2 (kendrin), pericentrin, kendrin (KIAA0402)10KENPCTN2, pericentrin-2, pericentrin-380, Pericentrin-B, SCKL4kendrin
Entrez Gene IDs
79902 (Human)
Gene Symbol
NUP85
UniProt
Additional Pericentrin Products
Product Documents for Pericentrin Antibody - C-terminus
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pericentrin Antibody - C-terminus
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Pericentrin Antibody - C-terminus (1)
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1 Customer Rating
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Application: ImmunofluorescenceSample Tested: RPE1 cellsSpecies: HumanVerified Customer | Posted 07/26/2021RPE1-hTERT cells are stained for pericentrin (Red) and primary cilia (Green). Pericentrin should be localized on the centrosome, base of the primary cilia. Based on the staining, there is no specific localization on the centrosome.IF done on 4% PFA (10min) and methanol (5min) fixed RPE1-hTERT cells. blocked with 5% BSA for 1hr at room temp. Primary antibodies: goat anti-pericentrin (1:100) and rabbit anti-Arl13B (1:500). Secondary antibodies: donkey anti-goat alexa fluor 555 and donkey anti-rabit alexa fluor 488.
Bio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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