Pericentrin Antibody - C-terminus

Name: Pericentrin Antibody - C-terminus
Brand: Novus Biologicals
SKU: NB100-63208
Price: 389 USD
Availability: InStock
Rating: 1 (1 reviews)

Novus Biologicals | Catalog # NB100-63208

(1)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Pericentrin Primary Antibodies »

Product Specifications

Immunogen

Peptide sequence C-ARAIIREGSLEGS corresponding to the C-terminal of pericentrin 1 (NP_079120)

Reactivity Notes

Predicted cross-reactivities: Mouse, Rat

Localization

Nuclear

Marker

Centrosome Marker

Specificity

NB100-63208 recognizes the C-terminal of human pericentrin 1, a 75 kDa component of the filamentous matrix of the centrosome, expressed in all tissues. When associated with centrosome and Golgi localized protein kinase N-associated protein (CG-NAP), pericentrin 1 anchors the gamma-tubulin ring complex, providing a site for microtubule nucleation and the initial establishment of organized microtubule arrays. It binds calmodulin and is a constituent of nuclear pore proteins (nup) 107-160, a sub-complex of the nuclear pore complex. Pericentrin 1 is thought to play a role in the functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Pericentrin Antibody - C-terminus

Western Blot: Pericentrin Antibody [NB100-63208]

Western Blot: Pericentrin Antibody [NB100-63208] - Staining of HeLa lysate (35 ug protein in RIPA buffer) using Goat anti Human pericentrin 1 antibody at 1.0 ug/mL. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Applications for Pericentrin Antibody - C-terminus

Application

Recommended Usage

ELISA

>1:32000

Western Blot

1 - 3 ug/mL

Reviewed Applications

Read 1 review rated 1 using NB100-63208 in the following applications:

Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TRIS buffered saline, 0.5% Bovine Serum Albumin

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pericentrin

Pericentrin is encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression.

Alternate Names

Kendrin, KIAA0402, MOPD2, PCN, PCNT2pericentrin B, PCNTB, pericentrin, pericentrin 2 (kendrin), pericentrin, kendrin (KIAA0402)10KENPCTN2, pericentrin-2, pericentrin-380, Pericentrin-B, SCKL4kendrin

Entrez Gene IDs

79902 (Human)

Gene Symbol

NUP85

UniProt

Q9BW27 (Human)

Additional Pericentrin Products

Product Documents for Pericentrin Antibody - C-terminus

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Certificate of Analysis

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Product Specific Notices for Pericentrin Antibody - C-terminus

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pericentrin Antibody - C-terminus (1)

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Pericentrin Antibody

Name: Anonymous

Application: Immunofluorescence

Sample Tested: RPE1 cells

Species: Human

Verified Customer | Posted 07/26/2021

RPE1-hTERT cells are stained for pericentrin (Red) and primary cilia (Green). Pericentrin should be localized on the centrosome, base of the primary cilia. Based on the staining, there is no specific localization on the centrosome.

IF done on 4% PFA (10min) and methanol (5min) fixed RPE1-hTERT cells. blocked with 5% BSA for 1hr at room temp. Primary antibodies: goat anti-pericentrin (1:100) and rabbit anti-Arl13B (1:500). Secondary antibodies: donkey anti-goat alexa fluor 555 and donkey anti-rabit alexa fluor 488.

Bio-Techne Response

This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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