Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82144

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Urine, Tissue Homogenate (100 uL)

Sensitivity

75 pg/mL

Assay Range

125 - 8000 pg/mL
View all Periostin/OSF-2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat POSTN/OSF-2 in samples. No Significant cross-reactivity or interference between Rat POSTN/OSF-2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 6%

Inter-Assay Precision (Precision between assays) CV% 6%

Recovery for Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

Recovery

average = 92% for serum; average = 97% for EDTA plasma; average = 93% for cell culture media

Linearity

Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

ELISA: Rat Periostin/OSF-2 ELISA Kit (Colorimetric) [NBP2-82144] -

ELISA: Rat Periostin/OSF-2 ELISA Kit (Colorimetric) [NBP2-82144] - Standard Curve Reference

Kit Contents for Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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