Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PERP Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human PERP. The immunogen is located within the last 50 amino acids of PERP. Amino Acid Sequence: EDDLLGNAKPRYFYTSA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Specificity

Human PERP has one isoform (193aa, 21kD). Mouse PERP has one isoform (193aa, 21.6kD) and Rat PERP also has one isoform (193aa, 21.6kD). NBP1-76741 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PERP Antibody - BSA Free

Western Blot: PERP AntibodyBSA Free [NBP1-76741]

Western Blot: PERP AntibodyBSA Free [NBP1-76741]

Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: PERP AntibodyBSA Free [NBP1-76741]

Western Blot: PERP AntibodyBSA Free [NBP1-76741]

Western Blot: PERP Antibody [NBP1-76741] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug lysates per lane. Antibodies: PERP NBP1-76741 (1ug/ml), PERP antibody from Company B (2 ug/ml), beta-actin NBP1-76692 (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: PERP AntibodyBSA Free [NBP1-76741]

Western Blot: PERP AntibodyBSA Free [NBP1-76741]

Western Blot: PERP Antibody [NBP1-76741] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PERP NBP1-76741 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
PERP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] -

Immunocytochemistry/ Immunofluorescence: PERP Antibody - BSA Free [NBP1-76741] - Validation of PERP in A431 Cells. Immunocytochemical analysis of A431 cells using anti-PEPR antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for PERP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PERP

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.

Alternate Names

dJ496H19.1, KCP1KCP-1, Keratinocyte-associated protein 1, KRTCAP11110017A08Rik, p53 apoptosis effector related to PMP22, p53 apoptosis effector related to PMP-22, P53-induced protein PIGPC1, PERP, TP53 apoptosis effector, PIGPC1RP3-496H19.1, THWkeratinocytes associated protein 1, Transmembrane protein THW

Gene Symbol

PERP

UniProt

np_071315 (Mouse)

Additional PERP Products

Product Documents for PERP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PERP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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