Phalloidin-TRITC

Tocris Bioscience | Catalog # 5783

Red-orange fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Tocris Bioscience

Key Product Details

Description

Red-orange fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
View all Fluorescent Actin Probes »

Product Description

Key information

: Phalloidin-TRITC is a red-orange fluorescent cytoskeleton stain. It binds and labels specifically F-actin.

Used for: Phalloidin-TRITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: fluorescent microscopy, immunofluorescence (IHC, ICC).

Properties and Photophysical Data: Phalloidin-TRITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Tetramethylrhodamine isothiocyanate (TRITC) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-TRITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 540 nm and 565 nm, respectively.

We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO.

For more information on how to use Phalloidin-TRITC, please see our protocol.

Scientific Data Images for Phalloidin-TRITC

Applications in HeLa Cells for Phalloidin-TRITC

Applications in HeLa Cells for Phalloidin-TRITC.

Application of Phalloidin-TRITC: HeLa cells were labeled with 150 nM Phalloidin-TRITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).

Spectra Viewer

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Product Specifications for Phalloidin-TRITC

Molecular Weight

1231.40

Formula

C60H70N12O13S2

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

7-[(4R)-5-[[[[4-[3,6-Bis(dimethylamino)xanthylium-9-yl]-3-carboxyphenyl]amino]thioxomethyl]amino]-4-hydroxy-L-leucine]phalloidin

CAS Number

915013-10-4

SMILES

C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C([O-])=O)=C(C=C2)C2=C3C=CC(C=C3OC3=C2C=CC(=C3)N(C)C)=[N+](C)C)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 3 publications citing the usage of this product.

There are currently no references for this product.

Product Documents for Phalloidin-TRITC

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Phalloidin-TRITC

For research use only

Citations for Phalloidin-TRITC

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  • Phalloidin TRITC
    Name: Chi Ngo
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: iPSC-derived cardiomyocytes
    Verified Customer | Posted 09/30/2025
    Phalloidin was added to the 2nd antibody solution and incubated with cells for 2h at RT.
    Phalloidin was used to label F-actin in iPSC-derived cardiomyocytes.
    Phalloidin-TRITC 5783

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