Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHAX Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 344 and 394 of human Phosphorylated Adaptor for RNA Export using the numbering given in entry NP_115553.2 (GeneID 51808).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHAX Antibody - BSA Free

Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268]

PHAX-Antibody-Western-Blot-NBP2-22268-img0004.jpg
Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody NBP2-22268 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268]

Western Blot: PHAX Antibody [NBP2-22268] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. NBP2-22268 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: PHAX Antibody [NBP2-22268]

Immunoprecipitation: PHAX Antibody [NBP2-22268]

Immunoprecipitation: PHAX Antibody [NBP2-22268] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22268 used for IP at 6 ug/mg lysate. PHAX was also immunoprecipitated by rabbit anti-PHAX antibody. For blotting immunoprecipitated PHAX, NBP2-22268 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for PHAX Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHAX

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA

Alternate Names

FLJ13193, phosphorylated adapter RNA export protein, phosphorylated adaptor for RNA export, RNA U small nuclear RNA export adapter protein, RNA U, small nuclear RNA export adapter (phosphorylation regulated), RNA U, small nuclear RNA export adaptor (phosphorylation regulated), RNUXA

Gene Symbol

PHAX

UniProt

NP_115553.2 (Human)

Additional PHAX Products

Product Documents for PHAX Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PHAX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PHAX Antibody - BSA Free

Customer Reviews for PHAX Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PHAX Antibody - BSA Free and earn rewards!

Have you used PHAX Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies