PHIP Antibody - BSA Free

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -Staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -

Immunohistochemistry-Paraffin: PHIP Antibody [NBP2-33883] -Staining of human liver shows no positivity in hepatocytes as expected.

Knockdown Validated: PHIP Antibody - BSA Free [NBP2-33883] -

DCAF14 regulates monomethylation of H4K20.(A) Whole-cell lysates were extracted from U2OS cells transfected with the indicated siRNAs. Immunoblots were probed with the antibodies as shown. PCNA serves as a loading control. (B) Whole-cell lysates were extracted from siNT- and siDCAF14-transfected U2OS cells. Immunoblots were probed with the several histone methylation antibodies as shown. PCNA serves as a loading control. (C) U2OS cells were either transfected with the indicated siRNAs or treated with SET8-inhibitor UNC0379 for 4 h. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. (D) siNT- and siDCAF14-transfected U2OS or HeLa cells were subjected to immunofluorescence analysis. Cells were immunostained for H4K20me1. Mean nuclei intensity was measured by quantitative imaging using DAPI-stained nuclei. Graphs represent mean +/- SEM using at least 450 nuclei. (E) Whole-cell lysates were extracted from siNT- and siDCAF14 (5′UTR)-transfected U2OS cells that were either mock transfected or overexpressing DCAF14. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. Graph represents normalized H4K20me1 intensities to histone H4 from three biological replicates.Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37940188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Knockdown Validated: PHIP Antibody - BSA Free [NBP2-33883] -

DCAF14 regulates monomethylation of H4K20.(A) Whole-cell lysates were extracted from U2OS cells transfected with the indicated siRNAs. Immunoblots were probed with the antibodies as shown. PCNA serves as a loading control. (B) Whole-cell lysates were extracted from siNT- and siDCAF14-transfected U2OS cells. Immunoblots were probed with the several histone methylation antibodies as shown. PCNA serves as a loading control. (C) U2OS cells were either transfected with the indicated siRNAs or treated with SET8-inhibitor UNC0379 for 4 h. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. (D) siNT- and siDCAF14-transfected U2OS or HeLa cells were subjected to immunofluorescence analysis. Cells were immunostained for H4K20me1. Mean nuclei intensity was measured by quantitative imaging using DAPI-stained nuclei. Graphs represent mean +/- SEM using at least 450 nuclei. (E) Whole-cell lysates were extracted from siNT- and siDCAF14 (5′UTR)-transfected U2OS cells that were either mock transfected or overexpressing DCAF14. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. Graph represents normalized H4K20me1 intensities to histone H4 from three biological replicates.Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37940188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Knockdown Validated: PHIP Antibody - BSA Free [NBP2-33883] -

DCAF14 prevents increased turnover of SET8.(A) Whole-cell lysates were extracted from U2OS, HeLa or hTERT-RPE1 cells transfected with the indicated siRNAs. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. (B) Whole cell lysates were extracted from siNT- and siDCAF14-transfected U2OS cells to analyze changes in SET8. Graph represents normalized SET8 intensities from four biological replicates. (C) Parental U2OS, DCAF14 KO, and DCAF14 cDNA-transfected KO cells were immunostained for SET8. Mean nuclei intensity was measured by quantitative imaging using DAPI-stained nuclei. Graphs represent mean +/- SEM using at least 3,500 nuclei. (D) Representative immunofluorescence images of siNT- and siDCAF14-transfected U2OS cells stained for DAPI, EdU, and SET8 are shown with overlay images. Scale bar = 10 μm. (E) siNT- and siDCAF14-transfected U2OS cells were pulsed with EdU for 30 min before immunofluorescence analyses. Mean nuclei intensity of SET8 was measured by quantitative imaging after preselecting EdU+ and EdU− nuclei. Graphs represent mean +/- SEM using at least 250 nuclei. (F) siNT- and siDCAF14-transfected U2OS cells were pretreated with either DMSO or MG132 for 2 h and pulsed with EdU during the last 30 min of treatment. Cells were immunostained for SET8 and mean nuclei intensity was measured by quantitative imaging after preselecting EdU+ and EdU− nuclei. Graphs represent mean +/- SEM using at least 250 nuclei.Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37940188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Knockdown Validated: PHIP Antibody - BSA Free [NBP2-33883] -

DCAF14 regulates monomethylation of H4K20.(A) Whole-cell lysates were extracted from U2OS cells transfected with the indicated siRNAs. Immunoblots were probed with the antibodies as shown. PCNA serves as a loading control. (B) Whole-cell lysates were extracted from siNT- and siDCAF14-transfected U2OS cells. Immunoblots were probed with the several histone methylation antibodies as shown. PCNA serves as a loading control. (C) U2OS cells were either transfected with the indicated siRNAs or treated with SET8-inhibitor UNC0379 for 4 h. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. (D) siNT- and siDCAF14-transfected U2OS or HeLa cells were subjected to immunofluorescence analysis. Cells were immunostained for H4K20me1. Mean nuclei intensity was measured by quantitative imaging using DAPI-stained nuclei. Graphs represent mean +/- SEM using at least 450 nuclei. (E) Whole-cell lysates were extracted from siNT- and siDCAF14 (5′UTR)-transfected U2OS cells that were either mock transfected or overexpressing DCAF14. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. Graph represents normalized H4K20me1 intensities to histone H4 from three biological replicates.Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37940188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Knockdown Validated: PHIP Antibody - BSA Free [NBP2-33883] -

DCAF14 regulates monomethylation of H4K20.(A) Whole-cell lysates were extracted from U2OS cells transfected with the indicated siRNAs. Immunoblots were probed with the antibodies as shown. PCNA serves as a loading control. (B) Whole-cell lysates were extracted from siNT- and siDCAF14-transfected U2OS cells. Immunoblots were probed with the several histone methylation antibodies as shown. PCNA serves as a loading control. (C) U2OS cells were either transfected with the indicated siRNAs or treated with SET8-inhibitor UNC0379 for 4 h. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. (D) siNT- and siDCAF14-transfected U2OS or HeLa cells were subjected to immunofluorescence analysis. Cells were immunostained for H4K20me1. Mean nuclei intensity was measured by quantitative imaging using DAPI-stained nuclei. Graphs represent mean +/- SEM using at least 450 nuclei. (E) Whole-cell lysates were extracted from siNT- and siDCAF14 (5′UTR)-transfected U2OS cells that were either mock transfected or overexpressing DCAF14. Immunoblots were probed with the antibodies as shown. KU70 serves as a loading control. Graph represents normalized H4K20me1 intensities to histone H4 from three biological replicates.Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37940188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.