Phospho-Axl (Y779) Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Phospho-Axl (Y779) in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 713610
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Phosphopeptide containing the human Axl Y779 site
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Phospho-Axl (Y779) in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line (A) resting or (B) treated with 100 nM Pervanadate for 10 minutes was stained with Mouse Anti-Human Phospho-Axl (Y779) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6965G) and Mouse Anti-Human Axl APC‑conjugated Monoclonal Antibody (Catalog # FAB154A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Phosphorylation of Tyrosine 779 provides a docking site for p85 subunits of PI 3-Kinase (4).

  • References:
    1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
    2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
    3. Holland, S. et al. (2005) Canc. Res. 65:9294.
    4. Weinger, J.G. et al. (2008) J. Neurochem. 106:134.
  • Long Name:
    Axl Receptor Tyrosine Kinase
  • Entrez Gene IDs:
    558 (Human); 26362 (Mouse)
  • Alternate Names:
    Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; EC 2.7.10; EC; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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