Phospho-CaM Kinase II (T286) Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat, Xenopus
  • Specificity
    Human, mouse, rat, bovine, chicken, Xenopus alpha -CaM Kinase II phosphorylated at T286 in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding phospho-T286 of CaM Kinase II
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-CaM Kinase II (T286) by Western Blot. Western Blot of rat brain tissue lysate showing specific immunolabeling of the ~50 kDa alpha -CaMKII subunit phosphorylated at T286 and the ~60 kDa beta -CaMKII subunit phosphorylated at T287 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the anti-phospho-CaMKII (T286). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: CaM Kinase II
Calmodulin Kinase II (CaMKII) is a 500 kDa, 8-12 subunit multimer that belongs to the Ser/Thr protein kinase family. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes ( alpha, beta, gamma, δ) that vary from 480-540 amino acids in length. The alpha - and beta -isozymes predominate in the brain. Each subunit contains a catalytic, autoregulatory, and subunit-association domain. The enzyme complex is inactive, due to the association of an internal pseudosubstrate motif with each subunit’s catalytic domain. CaMKII is regulated by calmodulin (CaM), an intracellular receptor for calcium. Following an influx of calcium, two Ca++-CaM complexes interact with inactive CaMKII at the autoregulatory site of two adjacent CaMKII subunits. This dissociates the catalytic site from the pseudosubstrate motif, allowing for the auto(cross)-phosphorylation of T286 on one alpha -subunit (T287 on a beta -subunit) by the catalytic site on an adjacent subunit. The T286 phosphorylation event blocks a reassociation of the catalytic domain with the internal pseudosubstrate motif, resulting in prolonged activation. Once activated, an autoinhibitory program ensues. The dissociation of Ca++-CaM from CaMKII exposes a Thr at position 305. CaMKII autophosphorylation of Thr at this site downregulates existing CaMKII activity.
  • References:
    1. Griffith, L.C. (2004) J. Neurosci. 24:8391.
    2. Hudmon, A. and H. Schulman (2002) Annu. Rev. Biochem. 71:473.
    3. Lin, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5962.
    4. Thiel, G. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6337.
    5. Elgersma, Y. et al. (2002) Neuron 36:493.
  • Long Name:
    Calcium/Calmodulin-dependent Protein Kinase II
  • Alternate Names:
    CaM Kinase II; CAMK2; CAMK2B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Ear2 deletion causes early memory and learning deficits in APP/PS1 mice.
    Authors: Kummer M, Hammerschmidt T, Martinez A, Terwel D, Eichele G, Witten A, Figura S, Stoll M, Schwartz S, Pape H, Schultze J, Weinshenker D, Heneka M, Urban I
    J Neurosci, 2014;34(26):8845-54.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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