Phospho-GluR1 (S831) Antibody PPS007: R&D Systems

Phospho-GluR1 (S831) Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Canine, Chicken
  • Specificity
    Specific for the ~100 kDa GluR1 protein phosphorylated at S831 in Western blots
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide corresponding to amino acid residues surrounding the phospho-S831 of GluR1
  • Formulation
    150 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Phospho-GluR1 (S831) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at S831 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-GluR1 (S831). The immunolabeling is completely eliminated by treatment with lambda -PPase.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GluR1
Rat GluR1 is a 907 amino acid, 4-transmembrane protein that belongs to the glutamate-gated ion channel family. It is one of four AMPA receptor subunits that form a functional heterotetrameric glutamate receptor. GluR1 only interacts with GluR2. GluR1 has two key serine residues in the C-terminal extracellular region. Serine 831 is constitutively unphosphorylated. Upon exposure to a neurotransmitter, it is phosphorylated by CaMKII leading to a potentiation of glutamate-mediated current. Serine 845 is constitutively phosphorylated by Protein Kinase A (PKA). The presence of the phosphate insures membrane localization of the subunit. Following NMDA receptor activation, S845 is dephosphorylated with subsequent subunit internalization.
  • Long Name:
    Glutamate Receptor 1
  • Entrez Gene IDs:
    2890 (Human); 14799 (Mouse); 50592 (Rat)
  • Alternate Names:
    AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR1; gluR-1; GLUR1gluR-A; GLURA; GluR-A; gluR-K1; glutamate receptor 1; Glutamate receptor ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA 1; GRIA1; HBGR1; MGC133252
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 42  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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