Phospholipase B1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93485

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Phospholipase B1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1110-1415 of human PLB1 (NP_694566.4). ARPNNSSDLPTSWRGLSWSIGGDGNLETHTTLPNILKKFNPYLLGFSTSTWEGTAGLNVAAEGARARDMPAQAWDLVERMKNSPDINLEKDWKLVTLFIGVNDLCHYCENPEAHLATEYVQHIQQALDILSEELPRAFVNVVEVMELASLYQGQGGKCAMLAAQNNCTCLRHSQSSLEKQELKKVNWNLQHGISSFSYWHQYTQREDFAVVVQPFFQNTLTPLNERGDTDLTFFSEDCFHFSDRGHAEMAIALWNNMLEPVGRKTTSNNFTHSRAKLKCPSPESPYLYTLRNSRLLPDQAEEAPEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

163 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phospholipase B1 Antibody - BSA Free

Western Blot: Phospholipase B1 AntibodyBSA Free [NBP2-93485]

Western Blot: Phospholipase B1 AntibodyBSA Free [NBP2-93485]

Western Blot: Phospholipase B1 Antibody [NBP2-93485] - Analysis of extracts of various cell lines, using Phospholipase B1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for Phospholipase B1 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phospholipase B1

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity includingphospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids anddiacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activitytoward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (Bysimilarity)

Alternate Names

FLJ30866, hPLB, phospholipase B1, PLBmembrane-associated

Gene Symbol

PLB1

Additional Phospholipase B1 Products

Product Documents for Phospholipase B1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Phospholipase B1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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