Phospholipase D2 Antibody (7E4D9) - BSA Free

Flow Cytometry: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

Flow Cytometry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).

Western Blot: Phospholipase D2 Antibody (7E4D9)BSA Free [NBP2-61784]

Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against HEK293 (1) and PLD2 (AA: 834-933)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/ Immunofluorescence: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of MCF-7 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded renal tissues using PLD2 mouse mAb with DAB staining.

ELISA: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

ELISA: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784] -

2‐DG inhibited glycolysis and mTOR pathway in OLP‐derived T cells. OLP‐derived T cells were activated with 1 μg/ml anti‐CD3 mAb and 2 μg/ml anti‐CD28 mAb for 72 h, then cultured with 1 mM or 5 mM 2‐DG for 24 h. 2‐DG was dissolved in DMSO. DMSO at a concentration of 0.1% (v/v) was used as the solvent control. (A) Representative images of immunofluorescent staining for LDHA expression in T cells. The fluorescence intensity of LDHA was decreased in the 2‐DG groups. 5 mM 2‐DG group showed a weaker fluorescence than 1 mM 2‐DG group. Red: LDHA. Blue: nucleus. Bar: 50 μm for ×400. (B) Representative images of Western Blot detection for p‐mTOR, mTOR, p‐4E‐BP1, 4E‐BP1, HIF1 alpha and PLD2 expression. Grey‐scale analysis revealed that the ratios of p‐mTOR/mTOR, p‐4E‐BP1/4E‐BP1, HIF1 alpha / beta ‐actin and PLD2/ beta ‐actin were decreased in 5 mM 2‐DG group, rather than in 1 mM 2‐DG group. ***, p < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34672419), licensed under a CC-BY license. Not internally tested by Novus Biologicals.