PHPT1 Antibody (007) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90109

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007 expressed in HEK293

Format

Azide and BSA Free
View all PHPT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human PHPT1 (Uniprot#: Q9NRX4-1; Ala2-Tyr125).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PHPT1 Antibody (007) - Azide and BSA Free

Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]

Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]

Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109] - Anti-PHPT1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: THP1 Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:14 kDa. Observed band size:14 kDa.
Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]

Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]

Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109] - PHPT1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-PHPT1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PHPT1 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size :14 kDa.

Applications for PHPT1 Antibody (007) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHPT1

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek et al., 2002 (PubMed 12383260)).(supplied by OMIM)

Alternate Names

bA216L13.10, DKFZp564M173, EC 3.1.3,14 kDa phosphohistidine phosphatase, EC 3.1.3.-, HSPC141, phosphohistidine phosphatase 11700008C22Rik, phosphohistidine phosphatase 14kDa, PHP14CGI-202, Protein janus-A homolog, RP11-216L13.10, sex-regulated protein janus-a

Gene Symbol

PHPT1

Additional PHPT1 Products

Product Documents for PHPT1 Antibody (007) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PHPT1 Antibody (007) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PHPT1 Antibody (007) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review PHPT1 Antibody (007) - Azide and BSA Free and earn rewards!

Have you used PHPT1 Antibody (007) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies