![Western Blot: PHRF1 Antibody [NBP1-18933]](https://resources.rndsystems.com/images/products/PHRF1-Antibody-Western-Blot-NBP1-18933-img0001.jpg "Western Blot: PHRF1 Antibody [NBP1-18933]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1400 and 1450 of human PHD and ring finger domains 1 using the numbering given in entry NP_065952.1 (GeneID 57661).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
179 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PHRF1 Antibody
Western Blot: PHRF1 Antibody [NBP1-18933]
Western Blot: PHRF1 Antibody [NBP1-18933] - Detection of Human and Mouse PHRF1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibodies: Affinity purified rabbit anti-PHRF1 antibody NBP1-18933 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. For blotting immunoprecipitated PHRF1, ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).Applications for PHRF1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PHRF1
Alternate Names
CTD binding SR like protein rA9, CTD-binding SR-like protein rA9, KIAA1542RNF221, PHD and RING finger domain-containing protein 1, PHD and ring finger domains 1
Gene Symbol
PHRF1
UniProt
Additional PHRF1 Products
Product Documents for PHRF1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PHRF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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