PIAS1 Antibody (3J2O8)

Novus Biologicals | Catalog # NBP3-16477

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3J2O8 expressed in HEK293
View all PIAS1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3-101 of human PIAS1 (O75925). DSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIAS1 Antibody (3J2O8)

Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477]

Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477]

Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477] - Western blot analysis of extracts of various cell lines, using PIAS1 Rabbit mAb (NBP3-16477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477]

Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477]

Western Blot: PIAS1 Antibody (3J2O8) [NBP3-16477] - Western blot analysis of extracts of Mouse lung, using PIAS1 Rabbit mAb (NBP3-16477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for PIAS1 Antibody (3J2O8)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIAS1

PIAS1 encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.

Long Name

Protein Inhibitor of Activated STAT 1

Alternate Names

DDXBP1, GU/RH-II, ZMIZ3

Gene Symbol

PIAS1

Additional PIAS1 Products

Product Documents for PIAS1 Antibody (3J2O8)

Certificate of Analysis

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Product Specific Notices for PIAS1 Antibody (3J2O8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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