PIEZO2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-61130

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human PIEZO2. Amino Acid Squence: HLTASLEKPEVRKLAEPGE

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Specificity

PIEZO2 Antibody is predicted to not cross-react with PIEZO1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

235 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIEZO2 Antibody - BSA Free

Western Blot: PIEZO2 AntibodyBSA Free [NBP2-61130]

Western Blot: PIEZO2 AntibodyBSA Free [NBP2-61130]

Western Blot: PIEZO2 Antibody [NBP2-61130] - Analysis of PIEZO2 in SK-N-SH cell lysate with PIEZO2 antibody at (A) 1 and (B) 2 ug/mL.
PIEZO2 Antibody - BSA Free

Western Blot: PIEZO2 Antibody - BSA Free [NBP2-61130] -

Western Blot: PIEZO2 Antibody - BSA Free [NBP2-61130] - WB Validation in Mouse Skeletal Muscle. Loading: 15 ug of mouse skeletal muscle lysate Antibodies: PIEZO2, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 min

Applications for PIEZO2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Simple Western

1:300

Western Blot

1-2 ug/ml
Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in Colon epithelial cell suspensions, separated by Size, antibody dilution of 1:300, apparent MW was 1:10000 kDa

Reviewed Applications

Read 1 review rated 4 using NBP2-61130 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIEZO2

Coming from the Greek ''piesi'' and meaning "pressure", PIEZO2 is integral to the membrane and is involved in ion channel activity, cation channel activity and the regulation of membrane potential. Having between 24 and 36 transmembrane domains, PIEZO's are big transmembrane proteins found in a variety of species. More specifically, PIEZO2 is required for rapidly adapting mechanically activated current in somatosensory and DRG neurons.

Long Name

PIEZO2

Alternate Names

C18orf30, C18orf58, DA3, DA5, DAIPT, FAM38B, FAM38B2, family with sequence similarity 38, member A pseud, family with sequence similarity 38, member B2, HsT748, HsT771, MWKS, piezo-type mechanosensitive ion channel component

Gene Symbol

PIEZO2

Additional PIEZO2 Products

Product Documents for PIEZO2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PIEZO2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PIEZO2 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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  • PIEZO2 Antibody
    Name: Moyad Almuslim
    Application: Western Blot
    Sample Tested: Trigeminal ganglia
    Species: Mouse
    Verified Customer | Posted 03/20/2020
    Western Blot: Piezo2 polyclonal antibody [NBP2-61130]. Analysis of mouse trigeminal ganglion cell lysates incubated with the antibody at 2µg/ml. Piezo1 band identified around 235kDa [the blue 250kDa and 180kDa markers can be seen].
    PIEZO2 Antibody - BSA Free NBP2-61130

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FAQs for PIEZO2 Antibody - BSA Free

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  • Q: In my project we need Anti Piezo2 and we find it in your catalogs. PIEZO2 Antibody. I want to know the prediction size of your antibody. The size of the protein is more than 200 kd. but in this photo there there are some sharp band are there the actual bands?

    A: The predicted molecular weights for this protein are based on the multiple transcripts associated with this protein. Please see Ensembl for detailed information. Those expected molecular weights are the following: 318.1, 80.8, 73.8, 62.7 kDa. We see this kind of staining across many of our antibodies for PIEZO2. It would appear that the transcript present is dependent on the tissue type. Based on protein arrays, we do believe that this antibody is specific.

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