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Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PIEZO2 Antibody - BSA Free
Western Blot: PIEZO2 AntibodyBSA Free [NBP2-61130]
Western Blot: PIEZO2 Antibody [NBP2-61130] - Analysis of PIEZO2 in SK-N-SH cell lysate with PIEZO2 antibody at (A) 1 and (B) 2 ug/mL.Western Blot: PIEZO2 Antibody - BSA Free [NBP2-61130] -
Western Blot: PIEZO2 Antibody - BSA Free [NBP2-61130] - WB Validation in Mouse Skeletal Muscle. Loading: 15 ug of mouse skeletal muscle lysate Antibodies: PIEZO2, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 minApplications for PIEZO2 Antibody - BSA Free
ELISA
Simple Western
Western Blot
See Simple Western Antibody Database for Simple Western validation: Tested in Colon epithelial cell suspensions, separated by Size, antibody dilution of 1:300, apparent MW was 1:10000 kDa
Reviewed Applications
Read 1 review rated 4 using NBP2-61130 in the following applications:
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Background: PIEZO2
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Additional PIEZO2 Products
Product Documents for PIEZO2 Antibody - BSA Free
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Product Specific Notices for PIEZO2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for PIEZO2 Antibody - BSA Free (1)
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Application: Western BlotSample Tested: Trigeminal gangliaSpecies: MouseVerified Customer | Posted 03/20/2020Western Blot: Piezo2 polyclonal antibody [NBP2-61130]. Analysis of mouse trigeminal ganglion cell lysates incubated with the antibody at 2µg/ml. Piezo1 band identified around 235kDa [the blue 250kDa and 180kDa markers can be seen].
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for PIEZO2 Antibody - BSA Free
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Q: In my project we need Anti Piezo2 and we find it in your catalogs. PIEZO2 Antibody. I want to know the prediction size of your antibody. The size of the protein is more than 200 kd. but in this photo there there are some sharp band are there the actual bands?
A: The predicted molecular weights for this protein are based on the multiple transcripts associated with this protein. Please see Ensembl for detailed information. Those expected molecular weights are the following: 318.1, 80.8, 73.8, 62.7 kDa. We see this kind of staining across many of our antibodies for PIEZO2. It would appear that the transcript present is dependent on the tissue type. Based on protein arrays, we do believe that this antibody is specific.