PIKFyve/PIP5K3 Antibody (6C7) - Azide and BSA Free

Novus Biologicals | Catalog # H00200576-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6C7

Format

Azide and BSA Free
View all PIKFyve Primary Antibodies »

Product Specifications

Immunogen

PIP5K3 (NP_689884, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR

Reactivity Notes

Human. Other species not tested.

Specificity

PIP5K3 - phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PIKFyve/PIP5K3 Antibody (6C7) - Azide and BSA Free

Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Immunocytochemistry/ Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01]

Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.

Applications for PIKFyve/PIP5K3 Antibody (6C7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIKFyve

PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization (Shisheva et al., 1999 [PubMed 9858586]).[supplied by OMIM]

Long Name

Phosphoinositide Kinase, FYVE Finger-containing

Alternate Names

FAB1, p235, PIP5K, PIP5K3, PIPkin-III

Entrez Gene IDs

200576 (Human)

Gene Symbol

PIKFYVE

OMIM

212 (Human)

UniProt

NP_689884 (Human)

Additional PIKFyve Products

Product Documents for PIKFyve/PIP5K3 Antibody (6C7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PIKFyve/PIP5K3 Antibody (6C7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PIKFyve/PIP5K3 Antibody (6C7) - Azide and BSA Free

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Protocols

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FAQs

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