Pin1 Antibody (3G8) - BSA Free

Novus Biologicals | Catalog # NBP1-22956

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G8

Format

BSA Free
View all Pin1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Pin1 (1-163 aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Pin1 Antibody (3G8) - BSA Free

Western Blot: Pin1 Antibody (3G8)BSA Free [NBP1-22956]

Western Blot: Pin1 Antibody (3G8)BSA Free [NBP1-22956]

Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow Cytometry: Pin1 Antibody (3G8) - BSA Free [NBP1-22956]

Flow Cytometry: Pin1 Antibody (3G8) - BSA Free [NBP1-22956]

Flow Cytometry: Pin1 Antibody (3G8) [NBP1-22956] - Analysis of Pin1 in Jurkat cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

Applications for Pin1 Antibody (3G8) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:500-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pin1

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Long Name

Protein [Peptidyl-prolyl cis/trans Isomerase] NIMA-interacting 1

Alternate Names

dod

Entrez Gene IDs

5300 (Human)

Gene Symbol

PIN1

UniProt

NP_006212 (Human)

Additional Pin1 Products

Product Documents for Pin1 Antibody (3G8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Pin1 Antibody (3G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

NFkB Pathway
p53 Pathway

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Protocols

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