PIP5K1C Antibody (PSH01-43)

Novus Biologicals | Catalog # NBP3-32824

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH01-43 expressed in HEK293
View all PIP5K1C Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human PIP5K1C aa 31-470 / 668. (Uniprot: O60331)

Localization

Cell membrane, Endomembrane system, Cytoplasm, Cell junction, focal adhesion, adherens junction, Cell projection, ruffle membrane, phagocytic cup, uropodium; Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIP5K1C Antibody (PSH01-43)

PIP5K1C Antibody (PSH01-43) Western Blot: PIP5K1C Antibody (PSH01-43) [NBP3-32824]

Western Blot: PIP5K1C Antibody (PSH01-43) [NBP3-32824]

Western blot analysis on different lysates with NBP3-32824 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: SiHa cell lysate Lane 6: A549 cell lysate Lane 7: LoVo cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 87/90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32824 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for PIP5K1C Antibody (PSH01-43)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIP5K1C

PIP5K1C encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus. [provided by RefSeq]

Long Name

Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma

Alternate Names

EC 2.7.1.68, KIAA0589, PIP5K1gamma

Gene Symbol

PIP5K1C

Additional PIP5K1C Products

Product Documents for PIP5K1C Antibody (PSH01-43)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PIP5K1C Antibody (PSH01-43)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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