PKC mu Antibody (S05-4J5)

Novus Biologicals | Catalog # NBP3-14964

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-4J5 expressed in HEK293
View all PRKD1/nPKC mu Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human PKC mu (Uniprot#: Q15139)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC mu Antibody (S05-4J5)

PKC mu Antibody (S05-4J5)

Western Blot: PKC mu Antibody (S05-4J5) [NBP3-14964] -

Western Blot: PKC mu Antibody (S05-4J5) [NBP3-14964] - Western blot detection of K562, C6, Hela cell lysates using NBP3-14964. Predicted band size: 102kDa. Observed band size: 102kDa.

Applications for PKC mu Antibody (S05-4J5)

Application
Recommended Usage

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

10mM PBS (pH 7.4), 150mM sodium chloride, 0.05% BSA, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRKD1/nPKC mu

PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).

Long Name

Protein Kinase D1

Alternate Names

PKCM, PRKD1

Gene Symbol

PRKD1

Additional PRKD1/nPKC mu Products

Product Documents for PKC mu Antibody (S05-4J5)

Certificate of Analysis

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Product Specific Notices for PKC mu Antibody (S05-4J5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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