Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B10

Format

BSA Free
View all PKM2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human PKM2 (1-531aa) purified from E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse PKM2 Antibody (1B10) - BSA Free (NBP2-42686) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PKM2 Antibody (1B10) - BSA Free

Western Blot: PKM2 Antibody (1B10)BSA Free [NBP2-42686]

Western Blot: PKM2 Antibody (1B10)BSA Free [NBP2-42686]

Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (1B10) - BSA Free [NBP2-42686]

Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (1B10) - BSA Free [NBP2-42686]

Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (1B10) - BSA Free [NBP2-42686]

Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (1B10) - BSA Free [NBP2-42686]

Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for PKM2 Antibody (1B10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKM2

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Long Name

Pyruvate Kinase, Muscle

Alternate Names

CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK

Gene Symbol

PKM

UniProt

NP_002645 (Human)

Additional PKM2 Products

Product Documents for PKM2 Antibody (1B10) - BSA Free

Certificate of Analysis

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Product Specific Notices for PKM2 Antibody (1B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

HIF Enhancer Pathways HIF Enhancer Pathway Thumbnail