PLAP Antibody (MSVA-350R)

Novus Biologicals | Catalog # NBP3-57232

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-350R
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Product Specifications

Immunogen

Recombinant full-length human ALPP protein Swissprot P05187

Localization

Cell Surface and Cytoplasmic

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Placenta (mature): A strong membranous and cytoplasmic staining of cytotrophoblast and syncytiotrophoblast must be seen..

Negative Control
Negitve control:Appendix: PLAP immunostaining must be absent in all structures.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PLAP Antibody (MSVA-350R)

PLAP Antibody (MSVA-350R)

Immunohistochemistry-Paraffin:PLAP Antibody (MSVA-350R)NBP3-57232

PLAP Placenta
PLAP Antibody (MSVA-350R)

Immunohistochemistry-Paraffin:PLAP Antibody (MSVA-350R)NBP3-57232

placenta mature amnion and chorion Zusatztext2
PLAP Antibody (MSVA-350R)

Immunohistochemistry-Paraffin:PLAP Antibody (MSVA-350R)NBP3-57232

Strong PLAP Immunostaining in all cells of a testicular seminoma.2 1

Applications for PLAP Antibody (MSVA-350R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PLAP

Phospholipase A2 activator protein (PLAP), activates PLA2 (phospholipase A2 enzyme) and is an important mediator of eicosanoid generation. Also known as a pro-inflammatory agent, PLAP has been found in inflamed tissues and synovial fluid from patients with rheumatoid arthritis. As such, it is believed to play an important role in the regulation of inflammatory diseases. The formation of PLAP can be stimulated by IL-1 beta and TNF-alpha (1-2).

Alternate Names

DOA1, FLJ12699, phospholipase A-2-activating protein, phospholipase A2-activating protein, PLAP

Gene Symbol

PLAA

Additional PLAP Products

Product Documents for PLAP Antibody (MSVA-350R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PLAP Antibody (MSVA-350R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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