Plasminogen Antibody - BSA Free

Novus Biologicals | Catalog # NB600-930

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Plasminogen Primary Antibodies »

Product Specifications

Immunogen

Plasminogen [Human Plasma] (Uniprot: P00747)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 15075185).

Localization

Secreted

Specificity

Cross reactivity against Plasminogen from other sources is unknown.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Plasminogen Antibody - BSA Free

Western Blot: Plasminogen Antibody [NB600-930]

Western Blot: Plasminogen Antibody [NB600-930]

Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.
Western Blot: Plasminogen Antibody [NB600-930]

Western Blot: Plasminogen Antibody [NB600-930]

Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.
Plasminogen Antibody

Plasminogen Antibody

Goat anti Plasminogen antibody ( lot 6571) was used to detect Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in ). Detection shown was using Dylight 649 conjugated Donkey anti goat ( lot 20834 1:10K in TBS/) 1 hr RT. Images were collected using the BioRad VersaDoc System.
Plasminogen Antibody

Plasminogen Antibody

Western blot of Goat Anti-Plasminogen primary antibody. Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.

Applications for Plasminogen Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:100000

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by western blot. This product is suitable to be assayed against 1.0 ug of Plasminogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.

Reviewed Applications

Read 2 reviews rated 5 using NB600-930 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plasminogen

Plasminogen is a plasma protein synthesised mostly in the liver. Plasma concentration is usually in the range of 70 to 200mg/L. Plasminogen is activated by tissue plasminogen activator, urokinase and streptokinase, to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen.

Alternate Names

Plg

Entrez Gene IDs

5340 (Human)

Gene Symbol

PLG

UniProt

P00747

Additional Plasminogen Products

Product Documents for Plasminogen Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Plasminogen Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Plasminogen Antibody - BSA Free

Customer Reviews for Plasminogen Antibody - BSA Free (2)

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  • Name: Catherine Brissette
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Other
    Verified Customer | Posted 03/03/2011
    Plasminogen Antibody - BSA Free NB600-930
  • Name: Ashutosh Verma
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Other
    Verified Customer | Posted 07/02/2010

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